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Browsing by Author "Pingoud A."

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Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis

Friedhoff P.; Kolmes B.; Gimadutdinow O.; Wende W.; Krause K.; Pingoud A. (1996)

Based on crystal structure analysis of the Serratia nuclease and a sequence alignment of six related nucleases, conserved amino acid residues that are located in proximity to the previously identified catalytic site residue ...
Biochemical characterization of Anabaena sp. strain PCC 7120 non- specific nuclease NucA and its inhibitor NuiA

Meiss G.; Franke I.; Gimadutdinow O.; Urbanke C.; Pingoud A. (1998)

We have established overexpression systems and purification protocols for NucA and NuiA, a sugar non-specific nuclease and its protein inhibitor from Anabaena sp. strain PCC 7120, in order to characterize these proteins ...
Chemical Rescue of Active Site Mutants of S. pneumoniae Surface Endonuclease EndA and Other Nucleases of the HNH Family by Imidazole

Midon M.; Gimadutdinow O.; Meiss G.; Friedhoff P.; Pingoud A. (2012)

The His-Asn-His (HNH) motif characterizes the active sites of a large number of different nucleases such as homing endonucleases, restriction endonucleases, structure-specific nucleases and, in particular, nonspecific ...
Experimental evidence for a ββα-Me-finger nuclease motif to represent the active site of the caspase-activated DNase

Scholz S.; Korn C.; Bujnicki J.; Gimadutdinow O.; Pingoud A.; Meiss G. (2003)

The caspase-activated DNase (CAD) is an important nuclease involved in apoptotic DNA degradation. Results of a sequence comparison of CAD proteins with ββα-Me-finger nucleases in conjunction with a mutational and chemical ...
Genetic engineering of Escherichia coli to produce a 1:1 complex of the Anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA

Korn C.; Meiss G.; Gast F.; Gimadutdinow O.; Urbanke C.; Pingoud A. (2000)

A series of T7-promoter based bicistronic expression vectors was constructed in order to produce the complex of the Anabaena sp. PCC 7120 DNA/RNA non-specific nuclease NucA and its inhibitor NuiA. With all constructs, ...
Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease

Franke I.; Meiss G.; Blecher D.; Gimadutdinow O.; Urbanke C.; Pingoud A. (1998)

The Serratia nuclease is a non-specific endonuclease which cleaves single- and double-stranded RNA and DNA. It is a member of a large family of related endonucleases, most of which fire dimers of identical subunits, with ...
Identification of functionally relevant histidine residues in the apoptotic nuclease CAD

Meiss G.; Scholz S.; Korn C.; Gimadutdinow O.; Pingoud A. (2001)

The caspase-activated DNase CAD (DFF40/CPAN) degrades chromosomal DNA during apoptosis. Chemical modification with DEPC inactivates the enzyme, suggesting that histidine residues play a decisive role in the catalytic ...
Interaction of DNA Fragmentation Factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state

Korn C.; Scholz S.; Gimadutdinow O.; Lurz R.; Pingoud A.; Meiss G. (2005)

DNA fragmentation factor (DFF) is a complex of the DNase DFF40 (CAD) and its chaperone/inhibitor DFF45 (ICAD-L) that can be activated during apoptosis to induce DNA fragmentation. Here, we demonstrate that DFF directly ...
Involvement of conserved histidine, lysine and tyrosine residues in the mechanism of DNA cleavage by the caspase-3 activated DNase CAD

Korn C.; Scholz S.; Gimadutdinow O.; Pingoud A.; Meiss G. (2002)

The caspase-activated DNase (CAD) is involved in DNA degradation during apoptosis. Chemical modification of murine CAD with the lysine-specific reagent 2,4,6-trinitrobenzenesulphonic acid and the tyrosine-specific reagent ...
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease

Friedhoff P.; Meiss G.; Kolmes B.; Pieper U.; Gimadutdinow O.; Urbanke C.; Pingoud A. (1996)

The extracellular nuclease from Serratia marcescens is a non-specific endonuclease that hydrolyzes double-stranded and single-stranded DNA and RNA with high specific activity. Steady-state and pre-steady-state kinetic ...
Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA

Meiss G.; Gimadutdinow O.; Haberland B.; Pingoud A. (2000)

A structural model of the DNA/RNA non-specific endonuclease NucA from Anabaena sp. PCC7120 that has been obtained on the basis of the three-dimensional structure of the related Serratia nuclease, suggests that the overall ...
Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis

Schäfer P.; Scholz S.; Gimadutdinow O.; Cymerman I.; Bujnicki J.; Ruiz-Carrillo A.; Pingoud A.; Meiss G. (2004)

Combining sequence analysis, structure prediction, and site-directed mutagenesis, we have investigated the mechanism of catalysis and substrate binding by the apoptotic mitochondrial nuclease EndoG, which belongs to the ...
The effect of ICAD-S on the formation and intracellular distribution of a nucleolytically active caspase-activated DNase

Scholz S.; Korn C.; Gimadutdinow O.; Knoblauch M.; Pingoud A.; Meiss G. (2002)

We show here that co-expression of murine CAD with either ICAD-L or ICAD-S in Escherichia coli as well as mammalian cells leads to a functional DFF complex, which after caspase-3 activation releases a nucleolytically active ...

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Browsing by Author "Pingoud A."

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