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Browsing by Author "Chastukhina I."

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Cloning and Heterologous Expression of Phytase Gene from Pantoea sp. 3.5.1

Suleimanova A.; Chastukhina I.; Valeeva L.; Itkina D.; Sharipova M. (2016)

© 2016, Springer Science+Business Media New York.Phytases (myo-inositol hexakisphosphate phosphohydrolase) catalyzes the stepwise hydrolysis of phosphate groups from phytic acid (myo-inositol hexakisphosphate) or its salt ...
Components of the ribosome biogenesis pathway underlie establishment of telomere length set point in Arabidopsis

Abdulkina L.; Kobayashi C.; Lovell J.; Chastukhina I.; Aklilu B.; Agabekian I.; Suescún A.; Valeeva L.; Nyamsuren C.; Aglyamova G.; Sharipova M.; Shippen D.; Juenger T.; Shakirov E. (2019)

© 2019, The Author(s). Telomeres cap the physical ends of eukaryotic chromosomes to ensure complete DNA replication and genome stability. Heritable natural variation in telomere length exists in yeast, mice, plants and ...
Heterologous expression of Bacillus intermedius gene of glutamyl endopeptidase in Bacillus subtilis strains defective in regulatory proteins

Shagimardanova E.; Chastukhina I.; Shamsutdinov T.; Balaban N.; Mardanova A.; Kostrov S.; Sharipova M. (2007)

Expression of the gene of glutamyl endopeptidase from Bacillus intermedius (gseBi) cloned on the plasmid pV has been studied in Bacillus subtilis recombinant strains with mutations of the regulatory proteins involved in ...
Inositol Phosphates and their Biological Effects

Balaban N.; Suleimanova A.; Valeeva L.; Chastukhina I.; Sharipova M. (2014)

The paper is dedicated to data analysis on inositol phosphates nature distribution, structure and biological functions as well as enzymes - phytases - capable to hydrolyze these hardly digestible compounds and their ...
Membrane-bound forms of serine proteases in Bacillus intermedius

Sharipova M.; Mardanova A.; Balaban N.; Gabdrakhmanova L.; Shilova M.; Chastukhina I.; Rudenskaya G.; Leshchinskaya I. (2003)

Proteolytic proteins solubilized from the membrane of Bacillus intermedius were studied by electrophoresis. The content of membrane-bound proteinases was lower in cells grown in the presence of glucose. Proteinase enzymograms ...
Membrane-bound forms of serine proteases in Bacillus intermedius

Sharipova M.; Mardanova A.; Balaban N.; Gabdrakhmanova L.; Shilova M.; Chastukhina I.; Rudenskaya G.; Leshchinskaya I. (2003)

Proteolytic proteins solubilized from the membrane of Bacillus intermedius were studied by electrophoresis. The content of membrane-bound proteinases was lower in cells grown in the presence of glucose. Proteinase enzymograms ...
Novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium Pantoea sp. strain 3.5.1

Suleimanova A.; Beinhauer A.; Valeeva L.; Chastukhina I.; Balaban N.; Shakirov E.; Greiner R.; Sharipova M. (2015)

© 2015, American Society for Microbiology. Phosphorus is an important macronutrient, but its availability in soil is limited. Many soil microorganisms improve the bioavailability of phosphate by releasing it from various ...
Optimization of cultivation media for heterologous gene expression of adamalysin-like melalloendopeptidase Bacillus pumilus

Rudakova N.; Balaban N.; Mardanova A.; Chastukhina I.; Sharipova M. (2015)

© 2015 Rudakova et al. The paper is dedicated to selecting main components of the nutrient medium and additional sources of nutrients for the maximum production of adamalysin-like metalloproteinase Bacillus pumilus. For ...
Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2005)

We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to ...
Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2005)

We studied the biosynthesis of Bacillus intermedius glutamyl endopeptidase in the recombinant Bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to ...
Selection of Efficient Taq DNA Polymerase to Optimize T-DNA Genotyping Method for Rapid Detection of Mutant Arabidopsis thaliana Plants

Chastukhina I.; Nigmatullina L.; Valeeva L.; Shakirov E. (2016)

© 2016, Springer Science+Business Media New York.Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other ...
Stable Co-Cultivation of the Moss Physcomitrella patens with Human Cells in vitro as a New Approach to Support Metabolism of Diseased Alzheimer Cells

Zakirova E.; Chastukhina I.; Valeeva L.; Vorobev V.; Rizvanov A.; Palotás A.; Shakirov E. (2019)

© 2019 - IOS Press and the authors. All rights reserved. Alzheimer's disease (AD) is a devastating slowly progressive neurodegenerative disorder with no cure. While there are many hypotheses, the exact mechanism causing ...
The expression of Bacillus intermedius glutamyl endopeptidase gene in Bacillus subtilis recombinant strains

Sharipova M.; Shagimardanova E.; Chastukhina I.; Shamsutdinov T.; Balaban N.; Mardanova A.; Rudenskaya G.; Demidyuk I.; Kostrov S. (2007)

The gene encoding for B. intermedius glutamyl endopeptidase (gseBi) has previously been cloned and its nucleotide sequence analyzed. In this study, the expression of this gene was explored in protease-deficient strain B. ...
The regulation of Bacillus intermedius glutamyl endopeptidase biosynthesis in the recombinant Bacillus subtilis strain AJ73 during sporulation

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Safina D.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2004)

The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 glutamyl endopeptidase gene on a multicopy plasmid and the effect of some nutrients on the efficiency of extracellular ...
The regulation of Bacillus intermedius glutamyl endopeptidase biosynthesis in the recombinant Bacillus subtilis strain AJ73 during sporulation

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Safina D.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2004)

The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 glutamyl endopeptidase gene on a multicopy plasmid and the effect of some nutrients on the efficiency of extracellular ...

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