P2 receptor subtypes in human hematopoietic cells of peripheral and cord blood

Abstract

P2 receptors have been found in several blood cells and their progenitors. However, most studies lack data about receptor subtypes and of receptors expression time in the process of cell differentiation. The aim of our study was to identify the subtypes of P2Y and P2X receptors on human CD34+ cells, c-kit+ cells, monocytes, lymphocytes of cord and peripheral blood. Expression of P2Y1, P2Y4, and P2Y6 receptors was the uniform in the cord and peripheral blood of all studied cells with the prevalence of monocytes expressing P2Y-receptors (up to 71%). At the same time, a significant difference was found between cells of cord and peripheral blood expressing subtypes of P2X2, P2X3, P2X4, P2X5, P2X6, P2X7 receptors. Cord blood lymphocytes contained a higher percentage of P2X receptors than peripheral blood lymphocytes. Similarly, the percentage of the peripheral blood monocytes, containing P2X receptors was significantly higher than the monocytes of cord blood.