Effect of Intermolecular Interactions in a Water/AOT/Isopropyl Myristate System on the Release of Biologically Active Substances

Abstract

Abstract: New biodegradable water/sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/isopropyl myristate (IPM) microemulsion systems for the delivery of medicinal and physiologically active substances are synthesized. The optimum concentrations of components that ensure solubilization of the largest amount of water-soluble substances are selected. The diffusion of model substances (L-lysine, L-serine, glycine, β-alanine) across a membrane is studied. It is found that the rate and pattern of release from the microemulsion vary strongly, depending on the terminal functional groups of amino acids. The effect the hydrophobicity and size of the introduced compounds have on the interaction with components of the transport system is determined using H1 NMR, self-diffusion NMR, the polarized luminescence of laurdan and fluorescein probes, and dynamic light scattering. It is shown that short-chain and polar amino acids are released much more quickly than charged amino acids. The results allow determination of the delivery characteristics of more complex counterparts (e.g., medicinal substances and protein molecules).