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Efficiency of RNA Hydrolysis by Binase from Bacillus pumilus: The Impact of Substrate Structure, Metal Ions, and Low Molecular Weight Nucleotide Compounds

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dc.contributor.author Kuznetsova A.A.
dc.contributor.author Akhmetgalieva A.A.
dc.contributor.author Ulyanova V.V.
dc.contributor.author Ilinskaya O.N.
dc.contributor.author Fedorova O.S.
dc.contributor.author Kuznetsov N.A.
dc.date.accessioned 2021-02-24T20:33:09Z
dc.date.available 2021-02-24T20:33:09Z
dc.date.issued 2020
dc.identifier.issn 0026-8933
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/160850
dc.description.abstract © 2020, Pleiades Publishing, Inc. Abstract: Binase is an extracellular guanyl-preferring ribonuclease from Bacillus pumilus. The main biological function of binase is RNA degradation with the formation of guanosine-2',3'-cyclic phosphate and its subsequent hydrolysis to 3'-phosphate. Extracellular RNases are believed to be key agents that affect the functional activity of the body, as they directly interact with epithelial and immune cells. The biological effects of the enzyme may consist of both direct RNA degradation, and the accumulation of 2',3'-cGMP in the human body. In this work, we have performed a comparative analysis of the cleavage efficiency of model RNA substrates, i.e., short hairpin structures that contain guanosine at various positions. It has been shown that the hydrolysis efficiency of the model RNA substrates depends on the position of guanosine. We have also demonstrated the influence of various divalent metal ions and low molecular weight nucleotide compounds on the binase-catalyzed endoribonucleolytic reaction.
dc.relation.ispartofseries Molecular Biology
dc.subject binase
dc.subject fluorescence
dc.subject pre-steady-state kinetics
dc.subject ribonuclease
dc.title Efficiency of RNA Hydrolysis by Binase from Bacillus pumilus: The Impact of Substrate Structure, Metal Ions, and Low Molecular Weight Nucleotide Compounds
dc.type Article
dc.relation.ispartofseries-issue 5
dc.relation.ispartofseries-volume 54
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 769
dc.source.id SCOPUS00268933-2020-54-5-SID85092796448


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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