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Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transfer

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dc.contributor.author Lomakin Y.
dc.contributor.author Kudryaeva A.
dc.contributor.author Kostin N.
dc.contributor.author Terekhov S.
dc.contributor.author Kaminskaya A.
dc.contributor.author Zakharova M.
dc.contributor.author Ivanova M.
dc.contributor.author Simaniv T.
dc.contributor.author Gabibov A.
dc.contributor.author Belogurov A.
dc.date.accessioned 2020-01-15T20:52:24Z
dc.date.available 2020-01-15T20:52:24Z
dc.date.issued 2019
dc.identifier.issn 0007-4888
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/155481
dc.description.abstract © 2019, Springer Science+Business Media, LLC, part of Springer Nature. Previous data showed that myelin-reactive autoantibodies found in patients with multiple sclerosis and mice with experimental autoimmune encephalomyelitis recognize and hydrolyze various fragments of myelin basic protein (MBP). Moreover, antibody-mediated cleavage of the encephalithogenic fragment MBP81-103 flanked with two fluorescent proteins can serve as a new biomarker of multiple sclerosis. Here we describe creation of the next generation of this biomarker based on antibody-dependent degradation of a new chemically synthesized fluorescent substrate with resonance energy transfer that contains fluorophore Cy5 and quencher QXL680 separated by MBP81-99 protein (Cy5-MBP81-99-QXL680). This substrate is degraded during incubation with purified antibodies and B cells from patients with multiple sclerosis, but not healthy volunteers.
dc.relation.ispartofseries Bulletin of Experimental Biology and Medicine
dc.subject antibodies
dc.subject B cells
dc.subject immunoglobulins
dc.subject myelin basic protein
dc.title Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transfer
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 167
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 329
dc.source.id SCOPUS00074888-2019-167-3-SID85069868341


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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