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dc.contributor.author | Deryagin O. | |
dc.contributor.author | Gavrilova S. | |
dc.contributor.author | Buravkov S. | |
dc.contributor.author | Andrianov V. | |
dc.contributor.author | Yafarova G. | |
dc.contributor.author | Gainutdinov K. | |
dc.contributor.author | Koshelev V. | |
dc.date.accessioned | 2018-09-19T22:12:36Z | |
dc.date.available | 2018-09-19T22:12:36Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1997-7298 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/144844 | |
dc.description.abstract | © 2016, Media Sphera. All rights reserved.Objective. To study a role of ATP-dependent potassium channels (K+ATP) in the neuroprotective effect of ischemic (IP) and pharmacological (PP) preconditioning and evaluate the dynamics of blood nitric oxide (NO) metabolites in cerebral ischemia. Material and methods. A model of ischemic stroke induced by the electrocoagulation of a middle cerebral artery (MCA) branch was used in male rats (n=86). Glibenclamide, a selective inhibitor of ATP-sensitive K+ channels, and diazoxide, a potassium channel activator, were used. IP and PP were performed 24 h before MCA occlusion. Blood concentrations of NO, NO3-and NO2-were measured 5, 24 and 72 h after occlusion. Results. IP decreased a lesion area by 37% (p<0/05) and the preliminary introduction ofglibenclamide levelled the effect of IP. A protective effect of PP was similar to that of IP. A decrease in oxygenated R-conformers of Hb-NO and a reverse increase in non-oxygenated T-conformers as well as NO3-и NO2-were noted 5h after MCA occlusion. In the first 24 h after MCA occlusion, contents of NO3-and NO2-returned to normal values. There were changes in the concentrations of Hb-NO complexes as well, with the predominance of R-conformers and minimal contents of T-conformers. Moreover, the correlations between K+ATP channel blockade and the decrease in serum NO3-and NO2 were found (p<0/03). Conclusion. The neuroprotective effect of preconditioning is caused by the activation of K+ATP channels. An analysis of NO metabolite concentrations in the blood of rats with IP suggests that Hb-NO complexes belonging to R-conformers deposit and carry NO in tissues releasing NO accumulated via R→T transfer in conditions of ischemia. | |
dc.relation.ispartofseries | Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova | |
dc.subject | ATP-dependent potassium channels | |
dc.subject | Cerebral ischemia | |
dc.subject | Electron paramagnetic resonance | |
dc.subject | Hemoglobin | |
dc.subject | Ischemic tolerance | |
dc.subject | Neuroprotection | |
dc.subject | Nitric oxide | |
dc.subject | Phenomenon of preconditioning | |
dc.subject | Spectrophotometry | |
dc.title | The role of ATP-dependent potassium channels and nitric oxide system in the neuroprotective effect of preconditioning | |
dc.type | Article | |
dc.relation.ispartofseries-issue | Special Issue 8 | |
dc.relation.ispartofseries-volume | 116 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 17 | |
dc.source.id | SCOPUS19977298-2016-116-8-SID85010788233 |