dc.contributor.author |
Deryagin O. |
|
dc.contributor.author |
Gavrilova S. |
|
dc.contributor.author |
Buravkov S. |
|
dc.contributor.author |
Andrianov V. |
|
dc.contributor.author |
Yafarova G. |
|
dc.contributor.author |
Gainutdinov K. |
|
dc.contributor.author |
Koshelev V. |
|
dc.date.accessioned |
2018-09-19T22:12:36Z |
|
dc.date.available |
2018-09-19T22:12:36Z |
|
dc.date.issued |
2016 |
|
dc.identifier.issn |
1997-7298 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/144844 |
|
dc.description.abstract |
© 2016, Media Sphera. All rights reserved.Objective. To study a role of ATP-dependent potassium channels (K+ATP) in the neuroprotective effect of ischemic (IP) and pharmacological (PP) preconditioning and evaluate the dynamics of blood nitric oxide (NO) metabolites in cerebral ischemia. Material and methods. A model of ischemic stroke induced by the electrocoagulation of a middle cerebral artery (MCA) branch was used in male rats (n=86). Glibenclamide, a selective inhibitor of ATP-sensitive K+ channels, and diazoxide, a potassium channel activator, were used. IP and PP were performed 24 h before MCA occlusion. Blood concentrations of NO, NO3-and NO2-were measured 5, 24 and 72 h after occlusion. Results. IP decreased a lesion area by 37% (p<0/05) and the preliminary introduction ofglibenclamide levelled the effect of IP. A protective effect of PP was similar to that of IP. A decrease in oxygenated R-conformers of Hb-NO and a reverse increase in non-oxygenated T-conformers as well as NO3-и NO2-were noted 5h after MCA occlusion. In the first 24 h after MCA occlusion, contents of NO3-and NO2-returned to normal values. There were changes in the concentrations of Hb-NO complexes as well, with the predominance of R-conformers and minimal contents of T-conformers. Moreover, the correlations between K+ATP channel blockade and the decrease in serum NO3-and NO2 were found (p<0/03). Conclusion. The neuroprotective effect of preconditioning is caused by the activation of K+ATP channels. An analysis of NO metabolite concentrations in the blood of rats with IP suggests that Hb-NO complexes belonging to R-conformers deposit and carry NO in tissues releasing NO accumulated via R→T transfer in conditions of ischemia. |
|
dc.relation.ispartofseries |
Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova |
|
dc.subject |
ATP-dependent potassium channels |
|
dc.subject |
Cerebral ischemia |
|
dc.subject |
Electron paramagnetic resonance |
|
dc.subject |
Hemoglobin |
|
dc.subject |
Ischemic tolerance |
|
dc.subject |
Neuroprotection |
|
dc.subject |
Nitric oxide |
|
dc.subject |
Phenomenon of preconditioning |
|
dc.subject |
Spectrophotometry |
|
dc.title |
The role of ATP-dependent potassium channels and nitric oxide system in the neuroprotective effect of preconditioning |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
Special Issue 8 |
|
dc.relation.ispartofseries-volume |
116 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
17 |
|
dc.source.id |
SCOPUS19977298-2016-116-8-SID85010788233 |
|