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Electrochemical aptasensor based on ZnO modified gold electrode

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dc.contributor.author Evtugyn G.
dc.contributor.author Cherkina U.
dc.contributor.author Porfireva A.
dc.contributor.author Danzberger J.
dc.contributor.author Ebner A.
dc.contributor.author Hianik T.
dc.date.accessioned 2018-09-18T20:13:24Z
dc.date.available 2018-09-18T20:13:24Z
dc.date.issued 2013
dc.identifier.issn 1040-0397
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/137707
dc.description.abstract We developed an electrochemical thrombin aptasensor based on ZnO nanorods functionalized by electrostatically adsorption of 30-mer DNA aptamers. The sensor surface was characterized by AFM and SEM. The surface layer assembling was optimized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with ferricyanide ions as redox markers. The peak current of the ferricyanide and the charge transfer resistance gradually decreased with increasing concentration of thrombin in the range from 3pM to 100nM due to formation of aptamer-thrombin complexes and slower diffusion of the marker ions through the surface layer. At optimal conditions, a limit of detection (LOD) of 3pM for EIS measurements and 10pM for CV response was calculated from the S/N=3. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.relation.ispartofseries Electroanalysis
dc.subject Aptasensor
dc.subject DNA Aptamer
dc.subject Electrochemical impedance spectroscopy
dc.subject Nanorods
dc.subject Thrombin
dc.subject Voltammetry
dc.subject Zinc oxide
dc.title Electrochemical aptasensor based on ZnO modified gold electrode
dc.type Article
dc.relation.ispartofseries-issue 8
dc.relation.ispartofseries-volume 25
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1855
dc.source.id SCOPUS10400397-2013-25-8-SID84881507346

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  • Публикации сотрудников КФУ Scopus [20180]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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