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dc.contributor.author | García-Alonsoa J. | |
dc.contributor.author | Fakhrullin R. | |
dc.contributor.author | Paunov V. | |
dc.date.accessioned | 2018-09-18T20:11:26Z | |
dc.date.available | 2018-09-18T20:11:26Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/137412 | |
dc.description.abstract | Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. © 2009 Elsevier B.V. | |
dc.relation.ispartofseries | Biosensors and Bioelectronics | |
dc.subject | Cell magnetization | |
dc.subject | GFP | |
dc.subject | Magnetic nanoparticles | |
dc.subject | Toxicity screening | |
dc.title | Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 7 | |
dc.relation.ispartofseries-volume | 25 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 1816 | |
dc.source.id | SCOPUS09565663-2010-25-7-SID77952306679 |