dc.contributor.author |
Cabrera-Fuentes H. |
|
dc.contributor.author |
Lopez M. |
|
dc.contributor.author |
McCurdy S. |
|
dc.contributor.author |
Fischer S. |
|
dc.contributor.author |
Meiler S. |
|
dc.contributor.author |
Baumer Y. |
|
dc.contributor.author |
Galuska S. |
|
dc.contributor.author |
Preissner K. |
|
dc.contributor.author |
Boisvert W. |
|
dc.date.accessioned |
2018-09-18T20:08:49Z |
|
dc.date.available |
2018-09-18T20:08:49Z |
|
dc.date.issued |
2015 |
|
dc.identifier.issn |
0340-6245 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/136966 |
|
dc.description.abstract |
© Schattauer 2015. Monocytes/macrophages respond to external stimuli with rapid changes in the expression of numerous inflammation-related genes to undergo polarisation towards the M1 (pro-inflammatory) or M2 (antiinflammatory) phenotype. We have previously shown that, independently of Toll-like receptor activation, extracellular RNA (eRNA) could exert pro-thrombotic and pro-inflammatory properties in the cardiovascular system to provoke cytokine mobilisation. Here, mouse bone marrow-derived-macrophages (BMDM) differentiated with mouse macrophage-colony-stimulating factor (M-CSF) were found to be skewed towards the M1 phenotype when exposed to eRNA. This resulted in up-regulated expression of inflammatory markers such as Tnf-α and Il-6, together with Il-12 and iNOS, whereas anti-inflammatory genes such as chitinase-like proteins (Ym1/2) and macrophage mannose receptor-2 (Cd206) were significantly down-regulated. Human peripheral blood monocytes were treated with eRNA and analysed by micro-array analysis of the whole human genome, revealing an up-regulation of 79 genes by at least four-fold; 27 of which are related to signal transduction and 15 genes associated with inflammatory response. In accordance with the proposed actions of eRNA as a pro-inflammatory “alarm signal”, these data shed light on the role of eRNA in the context of chronic inflammatory diseases such as atherosclerosis. |
|
dc.relation.ispartofseries |
Thrombosis and Haemostasis |
|
dc.subject |
Extracellular RNA |
|
dc.subject |
Gene expression |
|
dc.subject |
Inflammation |
|
dc.subject |
Microarray technology |
|
dc.subject |
Monocyte/macrophage polarization |
|
dc.title |
Regulation of monocyte/macrophage polarisation by extracellular RNA |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
3 |
|
dc.relation.ispartofseries-volume |
113 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
473 |
|
dc.source.id |
SCOPUS03406245-2015-113-3-SID84924193206 |
|