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dc.contributor.author | Fedorova K. | |
dc.contributor.author | Scharafutdinov I. | |
dc.contributor.author | Turbina E. | |
dc.contributor.author | Bogachev M. | |
dc.contributor.author | Ilinskaja O. | |
dc.contributor.author | Kayumov A. | |
dc.date.accessioned | 2018-09-18T20:06:14Z | |
dc.date.available | 2018-09-18T20:06:14Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0026-8933 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/136532 | |
dc.description.abstract | The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C-terminus controls the DNA-binding activity in a full-length TnrA. © 2013 Pleiades Publishing, Ltd. | |
dc.relation.ispartofseries | Molecular Biology | |
dc.subject | Bacillus subtilis | |
dc.subject | dimerization | |
dc.subject | transcription factor TnrA | |
dc.title | C-terminus of transcription factor TnrA from Bacillus subtilis controls DNA-binding domain activity but is not required for dimerization | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 2 | |
dc.relation.ispartofseries-volume | 47 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 293 | |
dc.source.id | SCOPUS00268933-2013-47-2-SID84876429954 |