Fluorescent polarization immunoassay for sulphadiazine using a high specificity antibody

dc.contributor.author	Murtazina N.
dc.contributor.author	Eremin S.
dc.contributor.author	Mozoleva O.
dc.contributor.author	Everest S.
dc.contributor.author	Brown A.
dc.contributor.author	Jackman R.
dc.date.accessioned	2018-09-17T20:56:21Z
dc.date.available	2018-09-17T20:56:21Z
dc.date.issued	2004
dc.identifier.issn	0950-5423
dc.identifier.uri	https://dspace.kpfu.ru/xmlui/handle/net/134284
dc.description.abstract	A rapid and sensitive fluorescent-labelled polarization immunoassay for sulphadiazine (SDZ) is reported. Fluorescein-labelled tracers were synthesized and a specific SDZ antibody has been used in the development of the method. The influence of the fluorescent label type on the immunoassay sensitivity was investigated. Homologous and non-homologous tracer - antiserum combinations were varied to provide increased sensitivity of the immunoassay. A detection limit of 0.2 ng mL-1 for SDZ in 50 μL aqueous sample has been achieved. The cross-reactivity of other sulphonamide drugs has also been examined.
dc.relation.ispartofseries	International Journal of Food Science and Technology
dc.subject	Competitive immunoassay
dc.subject	Fluorescent label
dc.title	Fluorescent polarization immunoassay for sulphadiazine using a high specificity antibody
dc.type	Article
dc.relation.ispartofseries-issue	8
dc.relation.ispartofseries-volume	39
dc.collection	Публикации сотрудников КФУ
dc.relation.startpage	879
dc.source.id	SCOPUS09505423-2004-39-8-SID8344255700

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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.