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Competitive immunochemical determination of antigens using conjugates containing Co(II) and Ni(II) | Konkurentnoe immunokhimicheskoe opredelenie antigenov s pomoshch'iu kon"iugatov, soderzhashchikh iony Co(II) i Ni(II).

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dc.contributor.author Dykhal I.
dc.contributor.author Mediantseva E.
dc.contributor.author Murtazina N.
dc.contributor.author Kalacheva N.
dc.contributor.author Budnikov G.
dc.date.accessioned 2018-09-17T20:45:56Z
dc.date.available 2018-09-17T20:45:56Z
dc.date.issued 2004
dc.identifier.issn 0555-1099
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/134014
dc.description.abstract A new variant of competitive heterogeneous immunoassay for certain proteinaceous antigens has been developed. The assay is based on the use of the target protein conjugated with Co(II) or Ni(II) ions and immobilized Abs. The effect of catalytic hydrogen release allows quantitation of the metal ion labels by voltammetry at the final step of the assay. The conjugates have been characterized by spectrophotometry, voltammetry, atomic adsorption spectrometry, and nuclear magnetic relaxation. Based on the use of the conjugate RNase-diethylenetriaminepentaacetic acid-Co(II) (10:4:4), a competitive immunoassay for RNase has been developed, detecting the target protein in the range 2 x 10(-2)-2 x 10(-4) mg/ml.
dc.relation.ispartofseries Prikladnaia biokhimiia i mikrobiologiia
dc.title Competitive immunochemical determination of antigens using conjugates containing Co(II) and Ni(II) | Konkurentnoe immunokhimicheskoe opredelenie antigenov s pomoshch'iu kon"iugatov, soderzhashchikh iony Co(II) i Ni(II).
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 40
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 236
dc.source.id SCOPUS05551099-2004-40-2-SID3142727861


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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