Kazan Federal University Digital Repository

Effect of a chemical analogue of autoinducers of microbial anabiosis on the Ca2+ response of mycelial fungi

Show simple item record

dc.contributor.author Kozlova O.
dc.contributor.author Kupriyanova-Ashina F.
dc.contributor.author Egorov S.
dc.contributor.author El'-Registan G.
dc.date.accessioned 2018-09-17T20:29:22Z
dc.date.available 2018-09-17T20:29:22Z
dc.date.issued 2004
dc.identifier.issn 0026-3656
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133562
dc.description.abstract The microbial alkylhydroxybenzenes (AHB), autoinducers of anabiosis, or d1 factors, participate in stress response of mycelial fungi, as determined from changes in intracellular Ca2+ concentration. By using the genetically modified strain Aspergillus awamori 66A, which produces a recombinant Ca2+-dependent protein aequorin, the dynamics of Ca 2+ was studied in the cytosol of cells exposed to mechanical shock in the presence of the protective doses (0.001-0.01% w/vol) of a chemical AHB analogue, 4-n-hexylresorcinol. Like under stressful conditions, Ca2+ concentration increases in the cell cytosol in response to enhanced AHB level in a growing fungal culture; thus, AHB is perceived by cells as a stress signal. The level of cell response, which was determined from the amplitude of luminescence dependent on the Ca2+ concentration in cytosol was related to the physiological age of the cells and AHB concentration. Micromycete preincubation with AHB was found to protect cells from subsequent stress; this was reflected in the Ca2+ response. The protective AHB effect was manifested as (1) a significant decrease in the amplitude of luminescence and, thus, in Ca2+ accumulation in the cytosol during subsequent mechanical stress (as compared to the control-mechanical stress only); (2) development of the secondary Ca2+ response, which was not observed in the control; (3) a high level of Ca2+ retained in the cytosol for a long time in the presence of AHB (as compared to the control without preincubation with AHB). The mechanisms underlying the AHB effect on the Ca 2+ transport systems are discussed.
dc.relation.ispartofseries Mikrobiologiya
dc.subject Alkylhydroxybenzenes
dc.subject Aspergillus awamori
dc.subject Ca2+ dynamics
dc.subject Mycelial fungi
dc.subject Protection from stress
dc.subject Recombinant aequorin
dc.subject Stress
dc.title Effect of a chemical analogue of autoinducers of microbial anabiosis on the Ca2+ response of mycelial fungi
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 73
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 741
dc.source.id SCOPUS00263656-2004-73-6-SID20444431898


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics