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Noncompetitive Immunochemical Determination of Ribonuclease Using Transition Metal Ions and the Effect of Catalytic Hydrogen Release

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dc.contributor.author Dykhal Y.
dc.contributor.author Medyantseva E.
dc.contributor.author Murtazina N.
dc.contributor.author Safina G.
dc.contributor.author Budnikov G.
dc.contributor.author Kalacheva N.
dc.date.accessioned 2018-09-17T20:06:09Z
dc.date.available 2018-09-17T20:06:09Z
dc.date.issued 2003
dc.identifier.issn 0003-6838
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133036
dc.description.abstract A noncompetitive variant of immunochemical ribonuclease (RNase) determination has been developed, involving the use of Co(II) as a label. A variety of approaches to labeling the immunological reagent with the metal have been assessed. In the variant proposed, catalytic hydrogen release is used as a means of detecting the label, the amount of which is proportional to the RNase concentration. Conditions making it possible to record catalytic hydrogen release currents have been determined. In the presence of RNase, the electrocatalytic effect is maximum at a concentration of Co(II) in ammoniac buffer equal to 2 × 10-4 M (pH 10.0). The dependence is linear in the RNase concentration range 2000-4 ng/ml (threshold concentration, 2 ng/ml).
dc.relation.ispartofseries Applied Biochemistry and Microbiology
dc.title Noncompetitive Immunochemical Determination of Ribonuclease Using Transition Metal Ions and the Effect of Catalytic Hydrogen Release
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 39
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 553
dc.source.id SCOPUS00036838-2003-39-6-SID0347285355


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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