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DNA hybridization on membrane-modified carbon electrodes

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dc.contributor.author Kouřilová A.
dc.contributor.author Babkina S.
dc.contributor.author Cahová K.
dc.contributor.author Havran L.
dc.contributor.author Jelen F.
dc.contributor.author Paleček E.
dc.contributor.author Fojta M.
dc.date.accessioned 2018-09-17T20:05:26Z
dc.date.available 2018-09-17T20:05:26Z
dc.date.issued 2005
dc.identifier.issn 0003-2719
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133016
dc.description.abstract The DNA-modified membrane electrode was prepared by casting a mixture of nitrocellulose (NC) with target DNA (tDNA) in organic solvent on glassy carbon electrode (GCE). Unlabeled polymerase chain reaction (PCR)-amplified human genomic sequence (628 bp) or synthetic oligodeoxynucleotides (ODNs) were used as tDNAs, creating a recognition layer. Biotinylated ODNs were used as hybridization probes to recognize specific nucleotide sequences. The hybridization events were detected via an enzyme-linked electrochemical assay involving binding of streptavidin-coupled alkaline phosphatase (SALP) to the biotin labels of the probe bound to tDNA. After the probe hybridization and SALP binding, the electrode was immersed into an electroinactive enzyme substrate (1-naphthyl phosphate). The alkaline phosphatase converted the inactive substrate into electroactive 1-naphthol that penetrated through the NC membrane to the GCE surface and was subsequently detected using an anodic voltammetric signal. The optimized method offered a good discrimination between complementary and nonspecific DNAs and yielded well-defined responses for both single-copy and repetitive tDNA sequences. In contrast to previously published methods using electrodes with mechanically attached membranes, the previously mentioned electrode is easily amenable to parallel DNA analysis. Copyright © Taylor & Francis, Inc.
dc.relation.ispartofseries Analytical Letters
dc.subject Alkaline phosphatase
dc.subject DNA hybridization
dc.subject Electrochemical DNA sensor
dc.subject Glassy carbon electrode
dc.subject Nitrocellulose membrane
dc.title DNA hybridization on membrane-modified carbon electrodes
dc.type Article
dc.relation.ispartofseries-issue 15
dc.relation.ispartofseries-volume 38
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 2493
dc.source.id SCOPUS00032719-2005-38-15-SID30744442514


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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