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Browsing by Author "Hartley R."

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Comparative study of thermostability and structure of close homologues - bamase and binase

Makarov A.; Protasevich I.; Kuznetsova N.; Fedorov B.; Korolev S.; Struminskaya N.; Bazhulina N.; Leshchinskaya 1.; Hartley R.; Kirpichnikov M.; Yakovlev G.; Esipova N. (1993)

Parameters of heat denaturation and intrinsic fluorescence of bamase and its close homologue, binase in the pH region 2-6 have been determined. The bamase heat denaturation (pH 2.85.5) proceeds according to the “all-or-none” ...
Contribution of arginine-82 and arginine-86 to catalysis of RNases from Bacillus intermedius (binase)

Yakovlev G.; Struminskaya N.; Znamenskaya L.; Kipenskaya L.; Leschinskaya I.; Hartley R. (1998)

To elucidate the functional role of Arg82 and Arg86 in the enzyme activity of binase, the extracellular ribonuclease of Bacillus intermedias, we used site-directed mutagenesis. On cleavage of various substrates the catalytic ...
Expression of the genes for guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus is regulated by the two component signal transduction system PhoP-PhoR in B. subtilis

Znamenskaya L.; Vershinina O.; Vershinina V.; Leshchinskaya I.; Hartley R. (1999)

Promoters of the genes for guanyl-specific ribonucleases, secreted by B. intermedius (binase) and B. pumilus (Rnase Bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in ...
Mutational analysis of the active site of RNase of Bacillus intermedius (BINASE)

Yakovlev G.; Moiseyev G.; Struminskaya N.; Borzykh O.; Kipenskaya L.; Znamenskaya L.; Leschinskaya I.; Chernokalskaya E.; Hartley R. (1994)

To elucidate the functional role of some residues in the active site of binase, the extracellular ribonuclease of Bacillus intermedius, we used site-directed mutagenesis. On cleavage of various substrates the catalytic ...
Phosphate regulation of biosynthesis of extracellular RNases of endospore-forming bacteria

Znamenskaya L.; Gabdrakhmanova L.; Chernokalskaya E.; Leshchinskaya I.; Hartley R. (1995)

The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P ...

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Browsing by Author "Hartley R."

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