Browsing by Author "Friedhoff P."

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Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis

Friedhoff P.; Kolmes B.; Gimadutdinow O.; Wende W.; Krause K.; Pingoud A. (1996)

Based on crystal structure analysis of the Serratia nuclease and a sequence alignment of six related nucleases, conserved amino acid residues that are located in proximity to the previously identified catalytic site residue ...
Chemical Rescue of Active Site Mutants of S. pneumoniae Surface Endonuclease EndA and Other Nucleases of the HNH Family by Imidazole

Midon M.; Gimadutdinow O.; Meiss G.; Friedhoff P.; Pingoud A. (2012)

The His-Asn-His (HNH) motif characterizes the active sites of a large number of different nucleases such as homing endonucleases, restriction endonucleases, structure-specific nucleases and, in particular, nonspecific ...
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease

Friedhoff P.; Meiss G.; Kolmes B.; Pieper U.; Gimadutdinow O.; Urbanke C.; Pingoud A. (1996)

The extracellular nuclease from Serratia marcescens is a non-specific endonuclease that hydrolyzes double-stranded and single-stranded DNA and RNA with high specific activity. Steady-state and pre-steady-state kinetic ...

Now showing items 1-3 of 3

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