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Browsing by Subject "Glutamyl endopeptidase"

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Effect of nutrients on the accumulation of glutamyl endopeptidase in the culture liquid of bacillus intermedium 3-19

Shakirov E.; Gabdrakhmanova L.; Balaban N.; Sharipova M.; Rudenskaya G.; Leshchinskaya I. (2000)

The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the culture liquid of Bacillus intermedius 3-19 was studied. Glucose and other readily metabolizable carbon sources were found ...
Effect of nutrients on the accumulation of glutamyl endopeptidase in the culture liquid of Bacillus intermedius 3-19

Shakirov E.; Gabdrakhmanova L.; Balaban N.; Sharipova M.; Rudenskaya G.; Leshchinskaya I. (2000)

The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the culture liquid of Bacillus intermedius 3-19 was studied. Glucose and other readily metabolizable carbon sources were found ...
Effect of the regulation system of metabolic nitrogen exchange on biosynthesis of serine proteinases from Bacillus intermedius

Kayumov A.; Shamsutdinov T.; Sabirova A.; Sharipova M. (2009)

The regulatory link between biosynthesis of Bacillus intermedius subtilisin-like serine proteinase and nitrogen metabolism in B. intermedius cells was determined. The level of the enzyme biosynthesis by the recombinant ...
Estimation of genotype, explant size and microbial enzymes influence on regenerative capacity of potatoes

Tikhonova O.; Toymentseva A.; Savenkova A.; Gizatullina A.; Gimaeva E.; Vologin S.; Karamova N.; Stasevski Z. (2015)

In this study effects of genotype, explants size and the composition of the culture medium on regeneration capacity of apexes of etiolated potato tubers sprouts were estimated. We observed positive correlation among survival, ...
Glutamyl endopeptidase of Bacillus intermedius, strain 3-19

Leshchinskaya I.; Shakirov E.; Itskovitch E.; Balaban N.; Mardanova A.; Sharipova M.; Viryasov M.; Rudenskaya G.; Stepanov V. (1997)

A homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic, rarely of aspartic acid residues in peptides and proteins, was isolated from Bacillus intermedius 3-19 culture filtrate using chromatography on CM ...
Heterologous expression of Bacillus intermedius gene of glutamyl endopeptidase in Bacillus subtilis strains defective in regulatory proteins

Shagimardanova E.; Chastukhina I.; Shamsutdinov T.; Balaban N.; Mardanova A.; Kostrov S.; Sharipova M. (2007)

Expression of the gene of glutamyl endopeptidase from Bacillus intermedius (gseBi) cloned on the plasmid pV has been studied in Bacillus subtilis recombinant strains with mutations of the regulatory proteins involved in ...
Hydrolytic enzymes and sporulation in Bacillus intermedius

Sharipova M.; Balaban N.; Gabdrakhmanova L.; Shilova M.; Kadyrova Y.; Rudenskaya G.; Leshchinskaya I. (2002)

The investigation of the activity of extracellular hydrolytic enzymes and sporulation in the bacterium Bacillus intermedius 3-19 showed that the activity of ribonuclease is maximal in the glucose-containing growth medium, ...
Isolation and characteristics of Bacillus intermedius glutamyl endopeptidase secreted by a recombinant strain of Bacillus subtilis at various growth phases

Shamsutdinov T.; Balaban N.; Mardanova A.; Danilova Y.; Rudeskaya G.; Sharipova M. (2008)

The recombinant strain of Bacillus subtilis bearing B. intermedius glutamyl endopeptidase gene in multicopy plasmid Δ58.21 secretes the enzyme to the medium at the phase of slowing of growth and the stationary growth phase ...
Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19

Balaban N.; Mardanova A.; Sharipova M.; Gabdrakhmanova L.; Sokolova E.; Garusov A.; Milgotina E.; Rudenskaya G.; Leshchinskaya I. (2003)

The culture filtrate of Bacillus intermedius 3-19 was used for isolation by chromatography on CM-cellulose and Mono S columns of a proteinase that is secreted during the late stages of growth. The enzyme is irreversibly ...
Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19

Balaban N.; Mardanova A.; Sharipova M.; Gabdrakhmanova L.; Sokolova E.; Garusov A.; Milgotina E.; Rudenskaya G.; Leshchinskaya I. (2003)

The culture filtrate of Bacillus intermedius 3-19 was used for isolation by chromatography on CM-cellulose and Mono S columns of a proteinase that is secreted during the late stages of growth. The enzyme is irreversibly ...
Membrane-bound forms of serine proteases in Bacillus intermedius

Sharipova M.; Mardanova A.; Balaban N.; Gabdrakhmanova L.; Shilova M.; Chastukhina I.; Rudenskaya G.; Leshchinskaya I. (2003)

Proteolytic proteins solubilized from the membrane of Bacillus intermedius were studied by electrophoresis. The content of membrane-bound proteinases was lower in cells grown in the presence of glucose. Proteinase enzymograms ...
Membrane-bound forms of serine proteases in Bacillus intermedius

Sharipova M.; Mardanova A.; Balaban N.; Gabdrakhmanova L.; Shilova M.; Chastukhina I.; Rudenskaya G.; Leshchinskaya I. (2003)

Proteolytic proteins solubilized from the membrane of Bacillus intermedius were studied by electrophoresis. The content of membrane-bound proteinases was lower in cells grown in the presence of glucose. Proteinase enzymograms ...
Optimization of Bacillus intermedius glutamyl endopeptidase production by recombinant strain of Bacillus subtilis and localization of glutamyl endopeptidase in Bacillus subtilis cells

Gabdrakhmanova L.; Balaban N.; Sharipova M.; Kostrov S.; Akimkina T.; Rudenskaya G.; Leshchinskaya I. (2002)

The biosynthesis of glutamyl endopeptidase from Bacillus intermedius 3-19 in recombinant strain of Bacillus subtilis has been investigated. The composition of culture medium, which yielded the maximum glutamyl endopeptidase ...
Optimization of cultivation medium for the production of Bacillus intermedius 3-19 glutamyl endopeptidase

Gabdrakhmanova L.; Balaban N.; Sharipova M.; Tokmakova Y.; Sokolova E.; Rudenskaya G.; Leshchinskaya I. (2002)

The effect of some components of cultivation medium on the growth of the streptomycin-resistant Bacillus intermedius strain 3-19 and on the production of glutamyl endopeptidase was investigated using factorial experimental ...
Optimized medium for the efficient production of bacillus intermedium glutamyl endopeptidase by the recombinant bacillus subtilis

Gabdrakhmanova L.; Shakirov E.; Balaban N.; Sharipova M.; Rudenskaya G. (2000)

A nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73 bearing the Bacillus intermedius 3-19 glutamyl endopeptidase gene within a multicopy ...
Optimized medium for the efficient production of Bacillus intermedium glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73

Gabdrakhmanova L.; Shakirov E.; Balaban N.; Sharipova M.; Rudenskaya G.; Kostrov S.; Akimkina T.; Leshchinskaya I. (2000)

A nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73 bearing the Bacillus intermedius 3-19 glutamyl endopeptidase gene within a multicopy ...
Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2005)

We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to ...
Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase

Chastukhina I.; Sharipova M.; Gabdrakhmanova L.; Balaban N.; Kostrov S.; Rudenskaya G.; Leshchinskaya I. (2005)

We studied the biosynthesis of Bacillus intermedius glutamyl endopeptidase in the recombinant Bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to ...
Proteinases from Bacillus intermedius secreted in the late stages of sporulation

Balaban N.; Gabdrakhmanova L.; Sharipova M.; Mardanova A.; Sokolova E.; Rudenskaya G.; Leshchinskaya I. (2002)

Background: Proteinases are widely used in various fields of medicine, such as the treatment of burns, purulent wounds, or decubitus ulcers. On the basis of new microbial proteinases produced by nonpathogenic organisms, a ...
Salt stress induction of glutamyl endopeptidase biosynthesis in Bacillus intermedius

Gabdrakhmanova L.; Vishniakov I.; Sharipova M.; Balaban N.; Kostrov S.; Leshchinskaya I. (2005)

Bacteria from the genus Bacillus have evolved complicated regulatory networks to be protected from various environmental stresses, including sudden increase in salinity. Among these regulatory mechanisms is the DegS-DegU ...

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Browsing by Subject "Glutamyl endopeptidase"

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